Cousins Bo and Luke Duke and their car "General Lee", assisted by Cousin Daisy and Uncle Jesse, have a running battle with the authorities of Hazzard County (Boss Hogg and Sheriff Coltrane), plus a string of ne'er-do-wells often backed by the scheming Hogg.
It's been 8 years since the Dukes got the General Lee and we see the story of how they got it. When it was originally used as a getaway car for some gold dust thieves and Bo and Luke got it from a junkyard because they needed a car to race in.
The Dukes' old friend Waylon Jennings comes to Hazzard with his mobile museum to use as a charity exhibit. But Boss hires two crooks to steal it and sell it back for a ransom.
Luke drinks some pond water that a truck accidentally spilled some genetic drugs into. This makes Luke character change a great deal. He becomes a total jerk, badmouths Bo, Daisy, and Jesse, harasses every girl at the Boar's Nest and eventually plans to rob Boss' bank.
A couple of thieves bring an advanced robot - that they stole for a ransom - through Hazzard County and Boss decides to buy it off them, fires Rosco, and make it the new sheriff: Robot P. Coltrane.
Boss swindles the Dukes into thinking they won a bunch of prizes at the Capitol City Department Store. All the prizes turn out to be stolen. However, Boss Hogg gets a bump on the head, later on, gets amnesia and becomes a nice gentleman - temporarily.
While a Hollywood production company is shooting a movie in Hazzard, the star almost has a car accident and the Dukes save him, and when the producers see how they saved him, they offer the Dukes a part in the movie and get to go to Hollywood for a while - but Boss and Rosco must go with them.
While driving through Osage, the most feared county around ruled by the mean Colonel Claiborne, Bo and Luke end up on a chain gang in the Osage Road Prison - which is the first step of Claiborne's plan to get Boss down there to swindle him out of everything he owns.
Boss Hogg finds a piece of paper from his great grandfather Thaddeus that Jesse's great grandfather Jeremiah signed over the Duke farm. Confused as ever, The Dukes find their great grandma's diary and they tell the story about the events leading up to it from the 1800's.
Cale Yarborough comes to Hazzard to see a sick boy in the hospital who is about to go in for surgery. But he and the Dukes ends up getting mixed up with bank robbers.
Boss Hogg masterminds a plan to knock a boxcar off a train containing valuables by hiring three crooks to hook a remote control up to a car to knock off the boxcar. But when the car they use is wrecked, they steal the General Lee to use it for the remote control.
Escape convicts come to Hazzard. One of them is out to settle a score with Luke because he sent him to prison in the Marines. Sheriff Little was put in charge of the investigation and the convicts force the Dukes to let them escape and end up driving the General Lee through a forest fire.
When a group of armored car robbers steals the armored car by lifting off the ground with a helicopter, the Dukes are blamed for the crime. But when Bo and Luke are apprehended, they strike again. Knowing that they're innocent, Boss and Rosco let the Duke boys escape so that they can do Rosco's dirty work and catch the robbers.
Boss' great uncle Silas Hogg had recently died and left in him a bunch of money in his will to give to charity at his discretion. Knowing that Boss won't give the money to charity, The Dukes set up some gadgets and make it look like Silus is haunting him into giving the money to charity.
Hughie Hogg returns to Hazzard with his ultimate plan to cheat Boss out of everything he owns by making him think there's a genie in a magic lamp that will give him all sorts of wishes.
With a lot of UFO sightings in Hazzard, Bo and Luke are starting to think that people are crazy until an alien from outer space hides in the General Lee and comes back to the Duke farm with them. However, when Boss finds out about this, he wants to take it and sell it.
A pair of crooks robs Boss' bank and frame Enos for it; Daisy becomes a witness against him when she thinks she saw it. Daisy, knowing that Enos would never do it, cannot refuse to testify against him or she'll be in contempt. So she decides to marry Enos - that way she cannot legally testify against him.
Boss and Lulu put on a talent show at the Boar's Nest. And when Rosco uses his magic act to make Boss disappear, he does when two parolees come to Hazzard who is out to kill Boss - who put them away. But instead they hold him for a ransom.